Human TLR9

Mouse

Human, Monkey, Mouse, Rat, Dog, Horse (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

FITC

Protein G purified

Unmodified

Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).

aa268-284

Recognizes human TLR9. Detects a band of ~120kD by Western blot, may also detect a smaller band of TLR9 isoform B.

• IHC
• IHC - Paraffin (5 - 10 µg/ml)
• IHC - Frozen (10 - 20 µg/ml)
• ICC
• Western blot (1 - 3 µg/ml)
• Flow Cytometry

Immunohistochemistry: LS-B2839 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2839 was determined to be 5-10 ug/ml. Flow Cytometry (Intracellular): 0.1-2 ug/10^6 cells; cell surface. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

10 mM Tris, 150 mM NaCl, 0.05% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9NR96 NCBI: NM_017442 NP_059138.1