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Catalog Number Size Price
LS-B2341-50 50 µg (1 mg/ml) $460 
TLR9 Antibody - Anti-TLR9 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TLR9 Antibody - Western blot of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using antibody at a dilution of 3 ug/ml. Lane E shows antibody tested at 5 ug/ml in human MCF7 cell lysate.
TLR9 Antibody - Intracellular flow analysis of TLR9 in Ramos cells using 0.1 ug of antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents anti-TLR9 antibody.
TLR9 Antibody - Anti-TLR9 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TLR9 Antibody - Western blot of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using antibody at a dilution of 3 ug/ml. Lane E shows antibody tested at 5 ug/ml in human MCF7 cell lysate.
TLR9 Antibody - Intracellular flow analysis of TLR9 in Ramos cells using 0.1 ug of antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents anti-TLR9 antibody.
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IHC‑plus™ Monoclonal Mouse anti‑Human TLR9 Antibody (clone 26C593.2, aa268‑284, IHC, WB) LS‑B2341

IHC‑plus™ Monoclonal Mouse anti‑Human TLR9 Antibody (clone 26C593.2, aa268‑284, IHC, WB) LS‑B2341

Note: This antibody replaces LS-C550
Antibody:
TLR9 Mouse anti-Human Monoclonal (aa268-284) (26C593.2) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B2341-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TLR9 Mouse anti-Human Monoclonal (aa268-284) (26C593.2) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TLR9 antibody LS-B2341 is an unconjugated mouse monoclonal antibody to TLR9 (aa268-284) from human. It is reactive with human, mouse and rat. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human TLR9
Synonyms
TLR9 | CD289 | CD289 antigen | Scri2a | Toll-like receptor 9
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
26C593.2
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9 isoform A (Genbank accession no. AAF78037). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Chimpanzee, Marmoset (94%); Sheep, Goat, Zebu, Bovine, Horse (82%).
Epitope
aa268-284
Specificity
KLH-conjugated synthetic peptide corresponding to amino acids 268-284 of human TLR9 isoform A (Genbank accession no. AAF78037).
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen (10 - 20 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2341 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2341 was determined to be 5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TLR9
Q9NR96 NM_017442 NP_059138.1

Validation

TLR9 Antibody - Anti-TLR9 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR9 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR9 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ TLR9 Antibody (aa268-284, clone 26C593.2) for IHC, WB/Western, Flow LS-B2341 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Association of self-DNA mediated TLR9-related gene, DNA methyltransferase, and cytokeratin protein expression alterations in HT29-cells to DNA fragment length and methylation status. Fűri I, Sipos F, Spisák S, Kiszner G, Wichmann B, Schöller A, Tulassay Z, Műzes G, Molnár B. TheScientificWorldJournal. 2014 2013:293296. (WB; Human)

Customer Reviews (0)


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Requested From: United States
Date Requested: 12/1/2024