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| Catalog Number | Size | Price |
|---|---|---|
| LS-A2666-50 | 50 µg (1 mg/ml) | $440 |
IHC‑plus™ Polyclonal Rabbit anti‑Human SMO / Smoothened Antibody (N‑Terminus, IHC, IF) LS‑A2666
IHC‑plus™ Polyclonal Rabbit anti‑Human SMO / Smoothened Antibody (N‑Terminus, IHC, IF) LS‑A2666
Antibody:
SMO / Smoothened Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Overview
Antibody:
SMO / Smoothened Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
Smoothened antibody LS-A2666 is an unconjugated rabbit polyclonal antibody to Smoothened (SMO) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 8 publications.
Target
Human SMO / Smoothened
Synonyms
SMO | Frizzled family member 11 | FZD11 | Gx | Protein Gx | SMOH | Smoothened | Smoothened homolog
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 15 amino acid peptide from N-Terminus extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%); Opossum (93%); Turkey, Chicken, Xenopus (87%); Zebrafish, Seq squirt (80%).
Epitope
N-Terminus
Specificity
Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- ICC
- Immunofluorescence
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SMO / Smoothened
Validation
Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
IHC-plus™ SMO / Smoothened Antibody (N-Terminus) for IHC, ICC, IF/Immunofluorescence LS-A2666 has an LSBio Rating of
Publications (4.7)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (8)
Vertebrate Smoothened functions at the primary cilium. Corbit KC, Aanstad P, Singla V, Norman AR, Stainier DY and Reiter JF. Nature. 2005 437:1018-21. (ICC; Mouse, Dog)
Sonic and desert hedgehog signaling in human fetal prostate development. Zhu G, Zhau HE, He H, Zhang L, Shehata B, Wang X, Cerwinka WH, Elmore J, He D. The Prostate. 2007 May;67:674-84.
Primary cilia modulate Ihh signal transduction in response to hydrostatic loading of growth plate chondrocytes. Shao YY, Wang L, Welter JF, Ballock RT. Bone. 2012 Jan;50:79-84.
Divergence of hedgehog signal transduction mechanism between Drosophila and mammals. Varjosalo M, Li SP, Taipale J. Developmental cell. 2009 Feb;10:177-86.
Primary cilia on porcine testicular somatic cells and their role in hedgehog signaling and tubular morphogenesis in vitro. Dores C, Alpaugh W, Su L, Biernaskie J, Dobrinski I. Cell and tissue research. 2017 April;368:215-223. (Human)
A role for primary cilia in aortic valve development and disease. Toomer KA, Fulmer D, Guo L, Drohan A, Peterson N, Swanson P, Brooks B, Mukherjee R, Body S, Lipschutz JH, Wessels A, Norris RA. Developmental dynamics : an official publication of the American Association of Anatomists. 2017 August;246:625-634.
Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence. Hehemann M, Kalmanek E, Choe S, Dynda D, Hu WY, Quek ML, Harrington DA, Stupp SI, McVary KT, Podlasek CA. Neurourology and urodynamics. 2018 November;37:2551-2559.
Rapamycin treatment correlates changes in primary cilia expression with cell cycle regulation in epithelial cells. Maha H Jamal, Ane C F Nunes, Nosratola D Vaziri, Ramani Ramchandran, Robert L Bacallao, Andromeda M Nauli, Surya M Nauli. Biochemical pharmacology. 2020 August;178:114056.
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Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/28/2024
Date Requested: 12/28/2024
