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Catalog Number Size Price
LS-B4911-50 50 µl (1 mg/ml) $460 
SMO / Smoothened Antibody - Human Small Intestine, Plasma: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Testis, Negative Signal: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Skin, Epidermis: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Breast, Lobule: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Prostate, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Western blot analysis of Anti-SMO antibody (LS-B4911, 1:500 dilution; 30 µg of protein per lane). Lane 1: HUVEC cell lysates. Antibody produced band at ~86 kDa. Lane 2: HUVEC cell lysates, antibody blocked with synthesized peptide. No Band produced with blocking.
SMO / Smoothened Antibody - Human Small Intestine, Plasma: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Testis, Negative Signal: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Skin, Epidermis: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Breast, Lobule: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Human Prostate, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
SMO / Smoothened Antibody - Western blot analysis of Anti-SMO antibody (LS-B4911, 1:500 dilution; 30 µg of protein per lane). Lane 1: HUVEC cell lysates. Antibody produced band at ~86 kDa. Lane 2: HUVEC cell lysates, antibody blocked with synthesized peptide. No Band produced with blocking.
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IHC‑plus™ Polyclonal Rabbit anti‑Human SMO / Smoothened Antibody (aa68‑117, IHC, WB) LS‑B4911

IHC‑plus™ Polyclonal Rabbit anti‑Human SMO / Smoothened Antibody (aa68‑117, IHC, WB) LS‑B4911

Note: This antibody replaces LS-C120950
Antibody:
SMO / Smoothened Rabbit anti-Human Polyclonal (aa68-117) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B4911-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SMO / Smoothened Rabbit anti-Human Polyclonal (aa68-117) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Smoothened antibody LS-B4911 is an unconjugated rabbit polyclonal antibody to Smoothened (SMO) (aa68-117) from human. It is reactive with human, mouse and rat. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human SMO / Smoothened
Synonyms
SMO | Frizzled family member 11 | FZD11 | Gx | Protein Gx | SMOH | Smoothened | Smoothened homolog
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human SMO. (AA Range: 68-117)
Epitope
aa68-117
Specificity
SMO Antibody detects endogenous levels of total SMO protein.
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot: Predicted MW 86 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMO / Smoothened
Q99835 NM_005631 NP_005622.1

Validation

SMO / Smoothened Antibody - Human Small Intestine, Plasma: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine, Plasma: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine, Plasma: Formalin-Fixed, Paraffin-Embedded (FFPE)

SMO / Smoothened Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

SMO / Smoothened Antibody - Human Testis, Negative Signal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis, Negative Signal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis, Negative Signal: Formalin-Fixed, Paraffin-Embedded (FFPE)

SMO / Smoothened Antibody - Human Skin, Epidermis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin, Epidermis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin, Epidermis: Formalin-Fixed, Paraffin-Embedded (FFPE)

SMO / Smoothened Antibody - Human Breast, Lobule: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast, Lobule: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast, Lobule: Formalin-Fixed, Paraffin-Embedded (FFPE)

SMO / Smoothened Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

SMO / Smoothened Antibody - Human Prostate, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ SMO / Smoothened Antibody (aa68-117) for IHC, WB/Western LS-B4911 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

Hedgehog signalling pathway activation in gastrointestinal stromal tumours is mediated by primary cilia. Pablo Iruzubieta, Marta Monzón, Tomás Castiella, Teresa Ramírez, Concepción Junquera. Gastric Cancer : official journal of the international Gastric Cancer Association and the Japanese Gastric Cancer Association. 2019 Jan;23:64-72.

Stromal cells of giant cell tumor of bone show primary cilia in giant cell tumor of bone. Tomás Castiella, Pablo Iruzubieta, Eva Monleón, Mª José Cardiel, Jesús Gómez-Vallejo, Marta Monzón, Mª Concepción Junquera. Microscopy research and technique. 2022 March;85:1065-1074.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/28/2024