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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1459-50 | 50 µl (85 mg/ml) | $480 |
IHC‑plus™ Polyclonal Rabbit anti‑Mouse Leptin Antibody (IHC, WB) LS‑B1459
IHC‑plus™ Polyclonal Rabbit anti‑Mouse Leptin Antibody (IHC, WB) LS‑B1459
Note: This antibody replaces LS-C19017
Antibody:
Leptin Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Leptin Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Specifications
Description
Leptin antibody LS-B1459 is an unconjugated rabbit polyclonal antibody to Leptin from mouse. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Mouse Leptin
Synonyms
LEP | Leptin | LEPD | Obese protein | Obese, mouse, homolog of | Obesity factor | OBS | OB
Host
Rabbit
Reactivity
Mouse, Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.
Specificity
The antibody will recognize recombinant and native 16 kDa leptin from mouse and human. Reactivity with leptin from other sources is unknown.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:2000)
- ELISA (1:1000 - 1:5000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1459 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1459 was determined to be 5 ug/ml.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About Leptin
Validation
Anti-LEP / Leptin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LEP / Leptin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ Leptin Antibody for IHC, WB/Western, ELISA LS-B1459 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Hypoplastic left heart syndrome is associated with structural and vascular placental abnormalities and leptin dysregulation. Jones HN, Olbrych SK, Smith KL, Cnota JF, Habli M, Ramos-Gonzales O, Owens KJ, Hinton AC, Polzin WJ, Muglia LJ, Hinton RB. Placenta. 2015 36:1078-86. (WB; Mouse)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
