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| Catalog Number | Size | Price |
|---|---|---|
| LS-B8192-500 | 500 µg (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human Leptin Antibody (IHC, WB) LS‑B8192
IHC‑plus™ Monoclonal Mouse anti‑Human Leptin Antibody (IHC, WB) LS‑B8192
Note: This antibody replaces LS-C104300, LS-C54171, LS-C89454, LS-C104734, LS-C25159
Antibody:
Leptin Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Leptin Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Leptin antibody LS-B8192 is an unconjugated mouse monoclonal antibody to human Leptin. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Leptin
Synonyms
LEP | Leptin | LEPD | Obese protein | Obese, mouse, homolog of | Obesity factor | OBS | OB
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human Leptin.
Specificity
Human Leptin
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (0.2 - 0.4 µg/ml)
- ELISA (12 - 16 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B8192 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant human Leptin when used with biotinylated antigen affinity purified anti-human Leptin as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2-4 ng/lane, under non-reducing conditions.
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About Leptin
Validation
Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
IHC-plus™ Leptin Antibody for IHC, WB/Western, ELISA LS-B8192 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Mouse, Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
