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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5747-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human IRS1 Antibody (aa289‑338, IHC, IF) LS‑B5747
IHC‑plus™ Polyclonal Rabbit anti‑Human IRS1 Antibody (aa289‑338, IHC, IF) LS‑B5747
Note: This antibody replaces LS-C117659
Antibody:
IRS1 Rabbit anti-Human Polyclonal (aa289-338) Antibody
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
IRS1 Rabbit anti-Human Polyclonal (aa289-338) Antibody
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
IRS1 antibody LS-B5747 is an unconjugated rabbit polyclonal antibody to IRS1 (aa289-338) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Target
Human IRS1
Synonyms
IRS1 | IRS-1 | HIRS-1 | Insulin receptor substrate 1
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human IRS-1. (AA Range: 289-338)
Epitope
aa289-338
Specificity
IRS1 Antibody detects endogenous levels of total IRS1 protein.
Applications
- IHC
- IHC - Paraffin (1:100)
- Immunofluorescence (1:100 - 1:500)
- Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blot: Predicted MW 131 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IRS1
Validation
Anti-IRS1 antibody IHC of human intestine, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-IRS1 antibody IHC of human intestine, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-IRS1 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-IRS1 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
See More About...
LSBio Ratings
IHC-plus™ IRS1 Antibody (aa289-338) for IHC, IF/Immunofluorescence LS-B5747 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Mouse, Human, Rat
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
Applications:
ICC, Western blot, Immunoprecipitation
![IRS1 Antibody - Anti-IRS1 pS307 Antibody - Western Blot. Western blot of Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kD believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kD is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/irs1-antibody-phospho-ser307-ls-c18824/62832_802708.jpg)
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
