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| Catalog Number | Size | Price |
|---|---|---|
| LS-B35-0.05 | 0.05 ml | $555 |
IHC‑plus™ Monoclonal Mouse anti‑Human IAPP / Amylin Antibody (clone R10/99, Supernatant, IHC, IF) LS‑B35
IHC‑plus™ Monoclonal Mouse anti‑Human IAPP / Amylin Antibody (clone R10/99, Supernatant, IHC, IF) LS‑B35
Note: This antibody replaces LS-C11532, LS-C68957, LS-C84778, LS-C20523, LS-C43301
Antibody:
IAPP / Amylin Mouse anti-Human Monoclonal (Supernatant) (R10/99) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF
Reactivity:
Human
Format:
Unconjugated, Supernatant
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
IAPP / Amylin Mouse anti-Human Monoclonal (Supernatant) (R10/99) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF
Reactivity:
Human
Format:
Unconjugated, Supernatant
Specifications
Description
Amylin antibody LS-B35 is an unconjugated mouse monoclonal antibody to human Amylin (IAPP). Validated for IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human IAPP / Amylin
Synonyms
IAPP | Diabetes-associated peptide | Islet amyloid polypeptide | AMYLIN | IAP | Insulinoma amyloid peptide
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
R10/99
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Supernatant
Immunogen
CATQRLANFLV coupled to tuberculin.
Specificity
Recognizes the islet amyloid polypeptide (amylin). Is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid.
Applications
- IHC
- IHC - Paraffin (1:100)
- IHC - Frozen
- Immunofluorescence
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B35 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B35 was determined to be 1:100.
Presentation
0.1M Tris, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IAPP / Amylin
Validation
Anti-Amylin antibody IHC of human pancreas, islet of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Amylin antibody IHC of human pancreas, islet of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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LSBio Ratings
IHC-plus™ IAPP / Amylin Antibody (clone R10/99, Supernatant) for IHC, IF/Immunofluorescence LS-B35 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Rational design of antibodies targeting specific epitopes within intrinsically disordered proteins. Pietro Sormanni, Francesco A Aprile, Michele Vendruscolo. Proceedings of the National Academy of Sciences of the United States of America. 2015 August;112:9902-7.
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Featured Products
Species:
Human, Rat, Cat, Guinea pig, Pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
