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Catalog Number	Size	Price
LS-B13122-50	50 µl (1 mg/ml)	$460

HSPA5 / GRP78 / BiP Antibody - Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPA5 / GRP78 / BiP Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPA5 / GRP78 / BiP Antibody - Immunofluorescent analysis of GRP78 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

HSPA5 / GRP78 / BiP Antibody - Western blot analysis of GRP78 expression in HepG2 (A); HeLa (B); Raw264.7 (C); mouse brain (D) whole cell lysates.

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IHC‑plus™ Polyclonal Rabbit anti‑Human HSPA5 / GRP78 / BiP Antibody (C‑Terminus, IHC, IF, WB) LS‑B13122

Name: IHC-plus™ HSPA5 / GRP78 / BiP Polyclonal Antibody Rabbit | LSBio
Brand: Lifespan BioSciences
SKU: LS-B13122-50
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C352339

Antibody:

HSPA5 / GRP78 / BiP Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Monkey, Mouse

Format:

Unconjugated, Unmodified

$460

LS-B13122-50

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For Research Use Only

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Overview

Antibody:

HSPA5 / GRP78 / BiP Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Monkey, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

BiP antibody LS-B13122 is an unconjugated rabbit polyclonal antibody to BiP (HSPA5 / GRP78) (C-Terminus) from human. It is reactive with human, mouse and monkey. Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human HSPA5 / GRP78 / BiP

Synonyms

HSPA5 | BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78

See All HSPA5 / GRP78 / BiP Antibodies

Host

Rabbit

Reactivity

Human, Monkey, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human GRP78.

Epitope

C-Terminus

Specificity

Recognizes endogenous levels of GRP78 protein.

Applications

IHC
IHC - Paraffin (1:100)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:10 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About HSPA5 / GRP78 / BiP

Uniprot: P11021 NCBI: M19645 AAA52614.1