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| Catalog Number | Size | Price |
|---|---|---|
| LS-C15345-50 | 50 µg (1 mg/ml) | $582 |
Monoclonal Mouse anti‑Rat HSPA5 / GRP78 / BiP Antibody (clone 10C3, aa649‑654, IHC, WB) LS‑C15345
Monoclonal Mouse anti‑Rat HSPA5 / GRP78 / BiP Antibody (clone 10C3, aa649‑654, IHC, WB) LS‑C15345
Antibody:
HSPA5 / GRP78 / BiP Mouse anti-Rat Monoclonal (aa649-654) (10C3) Antibody
Application:
IHC, ICC, WB, IP, ELISA
Reactivity:
Rat, Yeast
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
HSPA5 / GRP78 / BiP Mouse anti-Rat Monoclonal (aa649-654) (10C3) Antibody
Application:
IHC, ICC, WB, IP, ELISA
Reactivity:
Rat, Yeast
Format:
Unconjugated, Unmodified
Specifications
Description
BiP antibody LS-C15345 is an unconjugated mouse monoclonal antibody to BiP (HSPA5 / GRP78) (aa649-654) from rat. It is reactive with rat and yeast. Validated for ELISA, ICC, IHC, IP and WB.
Target
Rat HSPA5 / GRP78 / BiP
Synonyms
HSPA5 | BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78
Host
Mouse
Reactivity
Rat, Yeast
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
10C3
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A 6 residue synthetic peptide, SEKDEL, based on the rat Grp78 (aa 649-654), and the peptide coupled to KLH.
Epitope
aa649-654
Specificity
Identifies purified Grp78 (BiP) protein and reacts with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 on SDS-PAGE immunoblots in samples from mammal, bird, insect and plant origin. Cross reacts with yeast proteins containing the HDEL sequence. An unidentified protein at ~55kD is also detected in some lysates. This antibody does not react with Hsp70 (Hsp72) or Hsc70 (Hsp73).
Applications
- IHC (1:400)
- ICC (5 µg/ml)
- Western blot
- Immunoprecipitation (5 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot (Colorimetric): 4 ug/mL. Immunoprecipitation: 5 ug/mL. Immunocytochemistry: 5 ug/mL. Immunohistochemistry: 1:400. Positive control: HeLa Heat Shocked Cell Lysate.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HSPA5 / GRP78 / BiP
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Applications:
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Species:
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Species:
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Applications:
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Species:
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Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Reactivity:
Human
Range:
0.312-20 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/14/2024
Date Requested: 12/14/2024
