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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3905-50 | 50 µl | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human EPHB4 / EPH Receptor B4 Antibody (clone 5B5, Extracellular, IHC, IF, WB) LS‑B3905
IHC‑plus™ Monoclonal Mouse anti‑Human EPHB4 / EPH Receptor B4 Antibody (clone 5B5, Extracellular, IHC, IF, WB) LS‑B3905
Note: This antibody replaces LS-C108805, LS-C171459
Antibody:
EPHB4 / EPH Receptor B4 Mouse anti-Human Monoclonal (Extracellular) (5B5) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
EPHB4 / EPH Receptor B4 Mouse anti-Human Monoclonal (Extracellular) (5B5) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
EPH Receptor B4 antibody LS-B3905 is an unconjugated mouse monoclonal antibody to human EPH Receptor B4 (EPHB4) (Extracellular). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EPHB4 / EPH Receptor B4
Synonyms
EPHB4 | EPH receptor B4 | Ephrin receptor EphB4 | Ephrin type-B receptor 4 | HTK | MYK1 | TYRO11 | Soluble EPHB4 variant 1 | Soluble EPHB4 variant 3 | Hepatoma transmembrane kinase | MDK2 | Soluble EPHB4 variant 2 | Tyrosine-protein kinase TYRO11
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
5B5
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant extracellular fragment of human EPHB4 fused with hIgGFc tag expressed in HEK293 cell line.\
Epitope
Extracellular
Specificity
Human
Applications
- IHC
- IHC - Paraffin (1:200)
- Immunofluorescence (1:200 - 1:1000)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3905 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3905 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About EPHB4 / EPH Receptor B4
Validation
Anti-EPHB4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ EPHB4 / EPH Receptor B4 Antibody (Extracellular, clone 5B5) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B3905 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Species:
Mouse
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, Western blot, ELISA
Reactivity:
Mouse
Range:
31.2-2000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
