Target
Human EPHB4 / EPH Receptor B4
Synonyms
EPHB4 | EPH receptor B4 | Ephrin receptor EphB4 | Ephrin type-B receptor 4 | HTK | MYK1 | TYRO11 | Soluble EPHB4 variant 1 | Soluble EPHB4 variant 3 | Hepatoma transmembrane kinase | MDK2 | Soluble EPHB4 variant 2 | Tyrosine-protein kinase TYRO11
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
Recombinant protein derived from the C-Terminus of the human EphB4 protein.
Specificity
Recognizes human endogenous EphB4 receptor at ~110-120kD, with weak cross-reactivity with the EphB2 receptor.
Applications
- IHC
- IHC - Paraffin
- Western blot (1 - 3 µg/ml)
- Immunoprecipitation
- ELISA
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Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1-3 ug/ml. Immunoprecipitation: 5 ug/IP reaction. Immunohistochemistry: 10 ug/ml. Formalin-fixed, paraffin-embedded tissues require heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0 prior to staining. Positive control: Western Blot: EphB4-transfected 293T cells and human MDA-MB-231 breast carcinoma cell lysates.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EPHB4 / EPH Receptor B4