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Catalog Number Size Price
LS-B16765-100 100 µl $460 
CYLD Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CYLD Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CYLD Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
CYLD Antibody - Anti-CYLD Antibody at 1:4000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 107 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
CYLD Antibody - Overlay histogram showing Hela cells stained with CYLD Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (CYLD Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OJ192088) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2a (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
CYLD Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CYLD Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CYLD Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
CYLD Antibody - Anti-CYLD Antibody at 1:4000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 107 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
CYLD Antibody - Overlay histogram showing Hela cells stained with CYLD Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (CYLD Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OJ192088) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2a (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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IHC‑plus™ Monoclonal Mouse anti‑Human CYLD Antibody (clone 1667CT857.3.6.1, IHC, WB) LS‑B16765

IHC‑plus™ Monoclonal Mouse anti‑Human CYLD Antibody (clone 1667CT857.3.6.1, IHC, WB) LS‑B16765

Note: This antibody replaces LS-C757940
Antibody:
CYLD Mouse anti-Human Monoclonal (1667CT857.3.6.1) Antibody
Application:
IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16765-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CYLD Mouse anti-Human Monoclonal (1667CT857.3.6.1) Antibody
Application:
IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CYLD antibody LS-B16765 is an unconjugated mouse monoclonal antibody to human CYLD. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CYLD
Synonyms
CYLD | CDMT | Deubiquitinating enzyme CYLD | CYLDI | EAC | HSPC057 | MFT1 | MFT | SBS | TEM | BRSS | Ubiquitin thiolesterase CYLD | USPL2 | CYLD1 | KIAA0849 | Ubiquitin thioesterase CYLD
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
1667CT857.3.6.1
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
This CYLD antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 305-582 amino acids from human CYLD.
Applications
  • IHC - Paraffin (1:50)
  • Western blot (1:4000)
  • Flow Cytometry (1:25)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot Predicted Molecular Weight: 107.3 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYLD
Q9NQC7 NM_015247 CAB93533.1

Validation

CYLD Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

CYLD Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

CYLD Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ CYLD Antibody (clone 1667CT857.3.6.1) for IHC, WB/Western, Flow LS-B16765 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/25/2024