Horse CYCS / Cytochrome c

Mouse

Horse, Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

7H8.2C12

Unconjugated

Protein G purified

Unmodified

Horse cytochrome c.

Detects an ~14kD protein, corresponding to the apparent molecular mass of Cytochrome c on Western blots with samples from human, mouse, rat, bovine, guinea pig, hamster, monkey, rabbit and sheep origins. This antibody detects denatured Cytochrome c in applications such as Western blot, it does not recognize the native protein. The epitope of this antibody was mapped to a region within amino acids 93-104 of horse Cytochrome.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 µg/ml)

Immunohistochemistry: LS-B3673 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3673 was determined to be 5 ug/ml.

PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P99999 NCBI: NM_018947 NP_061820.1