IHC‑plus™ Monoclonal Mouse anti‑Pigeon CYCS / Cytochrome c Antibody (clone 7H8.2C12, aa1‑80, IHC, WB) LS‑B2141
IHC‑plus™ Monoclonal Mouse anti‑Pigeon CYCS / Cytochrome c Antibody (clone 7H8.2C12, aa1‑80, IHC, WB) LS‑B2141
Note: This antibody replaces LS-C514, LS-C36079, LS-C64190, LS-C87603, LS-C32998, LS-C36077, LS-C36078
Antibody:
CYCS / Cytochrome c Mouse anti-Pigeon Monoclonal (aa1-80) (7H8.2C12) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Pigeon, Human, Mouse, Rat, Hamster, Horse, Drosophila
Format:
Unconjugated, Unmodified
Overview
Antibody:
CYCS / Cytochrome c Mouse anti-Pigeon Monoclonal (aa1-80) (7H8.2C12) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Pigeon, Human, Mouse, Rat, Hamster, Horse, Drosophila
Format:
Unconjugated, Unmodified
Specifications
Description
Cytochrome c antibody LS-B2141 is an unconjugated mouse monoclonal antibody to Cytochrome c (CYCS) (aa1-80) from pigeon. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC and WB.
Target
Pigeon CYCS / Cytochrome c
Synonyms
CYCS | CYC | HCS | THC4 | Cytochrome c | Cytochrome c, somatic
Reactivity
Pigeon, Human, Mouse, Rat, Hamster, Horse, Drosophila
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Synthetic peptide from pigeon CYCS / Cytochrome c.
Specificity
An approx. 15 kD band is observed. This recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached).
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- ICC
- Western blot (1:500 - 1:1000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2141 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2141 was determined to be 5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYCS / Cytochrome c
Validation
Anti-Cytochrome c antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Cytochrome c antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Horse, Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit, Sheep
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Dog
Applications:
Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
Immunofluorescence, Immunoprecipitation, ELISA
Species:
Mouse, Human, Rat
Applications:
Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Horse, Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Reactivity:
Human
Range:
31.2-2000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024