Human CFH / Complement Factor H

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

OX-24

Unconjugated

Protein G purified

Unmodified

Human complement factor H.

Recognizes the human serum complement protein factor H (155kD), binding to a different epitope from MRC OX23. The Recognizes a 43-49kD truncated form of factor H present at low level (1-5 ug/ml) in plasma and urine. In an immunoprecipitation system. Recognizes factor H in other primates, but not in cow, sheep, pig, chicken or rabbit sera. Inhibits the binding of factor H to surface bound C3b, Recognizes Factor H in Western blotting procedures.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot
• Immunoprecipitation
• ELISA

• Flow Cytometry

Immunohistochemistry: LS-B3648 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3648 was determined to be 10 ug/ml.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08603 NCBI: NM_000186 NP_000177.2