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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2585-50 | 50 µg (0.5 mg/ml) | $485 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Goat anti‑Human CFH / Complement Factor H Antibody (aa577‑588, IHC, WB) LS‑B2585
IHC‑plus™ Polyclonal Goat anti‑Human CFH / Complement Factor H Antibody (aa577‑588, IHC, WB) LS‑B2585
Note: This antibody replaces LS-C54667, LS-C65824
Antibody:
CFH / Complement Factor H Goat anti-Human Polyclonal (aa577-588) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CFH / Complement Factor H Goat anti-Human Polyclonal (aa577-588) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Complement Factor H antibody LS-B2585 is an unconjugated goat polyclonal antibody to human Complement Factor H (CFH) (aa577-588). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CFH / Complement Factor H
Synonyms
CFH | Adrenomedullin binding protein | AHUS1 | AMBP1 | ARMS1 | ARMD4 | Beta-1-H-globulin | Factor H | Factor H-like 1 | HF | HF2 | H factor 1 | H factor 2 (complement) | HUS | HF1 | Beta-1H | CFHL3 | Complement factor H | H factor 1 (complement)
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence according to NP_000177.2.
Epitope
aa577-588
Specificity
Human CFH / Factor H. This antibody is expected to recognize isoform a (NP_000177.2) only.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2585 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2585 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CFH / Complement Factor H
Validation
Anti-Factor H antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Factor H antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CFH / Complement Factor H Antibody (aa577-588) for IHC, WB/Western LS-B2585 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human
Applications:
Flow Cytometry
Species:
Human
Applications:
Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Mouse, Rat
Applications:
IHC, IHC - Frozen, Western blot
Reactivity:
Mouse
Range:
7.8-500 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/19/2024
Date Requested: 11/19/2024
