Target
Human CDK2
Synonyms
CDK2 | Cell division protein kinase 2 | CDC2-related protein kinase | p33 protein kinase | p33(CDK2) | CDKN2 | Cyclin-dependent kinase 2
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG2a + IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Human recombinant cdk2 protein.
Specificity
Recognizes human Cdk2. Species cross-reactivity: mouse and rat.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence
- Western blot (1 - 2 µg/ml)
- Immunoprecipitation
- ELISA
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2482 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2482 was determined to be 5 ug/ml. Immunoprecipitation: 2 ug/mg protein lysate. (Denatured only) Use Protein A. Western Blot: 1-2 ug/ml for 2hrs at RT. Positive control: HeLa cells.
Presentation
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 40% Glycerol, 0.2% BSA
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee