Target
Human CDK2
Synonyms
CDK2 | Cell division protein kinase 2 | CDC2-related protein kinase | p33 protein kinase | p33(CDK2) | CDKN2 | Cyclin-dependent kinase 2
Reactivity
Human, Mouse, Xenopus
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Recombinant human cdk2.
Specificity
Recognizes human cdk2, MW 33kD. Does not cross-react with other cdks. Species cross-reactivity: mouse and Xenopus.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
- Immunoprecipitation
- ELISA
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2483 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2483 was determined to be 5 ug/ml.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee