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Catalog Number Size Price
LS-B2001-50 50 µg (0.5 mg/ml) $460 
CD275 / B7-H2 / ICOS Ligand Antibody - Anti-ICOSLG / ICOSL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
CD275 / B7-H2 / ICOS Ligand Antibody - Human monocyte-derived dendritic cells stained with purified 2D3, then detected with biotinylated anti-mouse IgGs and Sav-PE. Antibody used ad 0.5 ug/million cells in 100 ul volume.
CD275 / B7-H2 / ICOS Ligand Antibody - Anti-ICOSLG / ICOSL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
CD275 / B7-H2 / ICOS Ligand Antibody - Human monocyte-derived dendritic cells stained with purified 2D3, then detected with biotinylated anti-mouse IgGs and Sav-PE. Antibody used ad 0.5 ug/million cells in 100 ul volume.
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IHC‑plus™ Monoclonal Mouse anti‑Human CD275 / B7‑H2 / ICOS Ligand Antibody (clone 2D3, IHC) LS‑B2001

IHC‑plus™ Monoclonal Mouse anti‑Human CD275 / B7‑H2 / ICOS Ligand Antibody (clone 2D3, IHC) LS‑B2001

Note: This antibody replaces LS-C41124
Antibody:
CD275 / B7-H2 / ICOS Ligand Mouse anti-Human Monoclonal (2D3) Antibody
Application:
IHC, IHC-P, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B2001-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD275 / B7-H2 / ICOS Ligand Mouse anti-Human Monoclonal (2D3) Antibody
Application:
IHC, IHC-P, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ICOS Ligand antibody LS-B2001 is an unconjugated mouse monoclonal antibody to human ICOS Ligand (CD275 / B7-H2). Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human CD275 / B7-H2 / ICOS Ligand
Synonyms
ICOSLG | B7RP1 | B7 homolog 2 | B7 homologue 2 | B7-H2 | B7H2 | B7RP-1 | CD275 antigen | ICOS ligand | GL50 | ICOS-L | ICOSL | KIAA0653 | B7-like protein Gl50 | B7-related protein 1 | CD275 | LICOS
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
2D3
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Full-length human B7H2-mIg fusion protein.
Specificity
Human ICOSL/CD275/B7H2
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Flow Cytometry (0.5 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2001 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2001 was determined to be 1:50.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide
Storage
Store at 4°C. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD275 / B7-H2 / ICOS Ligand
O75144 AF289028 AAG01176.1

Validation

CD275 / B7-H2 / ICOS Ligand Antibody - Anti-ICOSLG / ICOSL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-ICOSLG / ICOSL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-ICOSLG / ICOSL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

LSBio Ratings

IHC-plus™ CD275 / B7-H2 / ICOS Ligand Antibody (clone 2D3) for IHC, Flow LS-B2001 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/11/2024