Target
Human CD275 / B7-H2 / ICOS Ligand
Synonyms
ICOSLG | B7RP1 | B7 homolog 2 | B7 homologue 2 | B7-H2 | B7H2 | B7RP-1 | CD275 antigen | ICOS ligand | GL50 | ICOS-L | ICOSL | KIAA0653 | B7-like protein Gl50 | B7-related protein 1 | CD275 | LICOS
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rhICOS Ligand.
Specificity
Recognizes human ICOS Ligand. In direct ELISAs and Western Blots, this antibody shows no cross-reactivity with rhB7-1, rhB7-2, rhB7-H1, rhB7-H3, rmB7-H2 or rmPD-L2.
Applications
- Western blot (1 - 2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
- Neutralization
Usage
Suitable for use in Neutralization, ELISA and Western Blot. Neutralization: ~1-4 ug/ml Dependent on cell type, growth conditions, activity studied. ND50 in the presence of 20 ng/ml of anti-human CD3 and 3 ug/ml of recombinant human B7-H2/Fc, using B7-H2-dependent proliferation of PHA stimulated human T cells as an assay. ELISA: 0.5-1 ug/ml, limit B7-H2 ~5 ng/well. Western Blot: 1-2 ug/ml, limit B7-H2 ~1 ng/lane.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Phosphate Buffered Saline (PBS)
Storage
Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD275 / B7-H2 / ICOS Ligand