Human CD275 / B7-H2 / ICOS Ligand

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived rhICOS Ligand.

Recognizes human ICOS Ligand. In direct ELISAs and Western Blots, this antibody shows no cross-reactivity with rhB7-1, rhB7-2, rhB7-H1, rhB7-H3, or rmB7-H2.

• Western blot (1 - 2 µg/ml)
• Flow Cytometry (25 µg/ml)
• ELISA (0.5 - 1 µg/ml)
• Neutralization

Suitable for use in Neutralization, Flow Cytometry, Western Blot and Direct ELISA. Neutralization: ~1-4 ug/ml Dependent on cell type, growth conditions, activity studied. ND50 in the presence of 20 ng/mL of anti-human CD3 and 3 ug/ml of recombinant human B7-H2/Fc, using 72 hours PHA-stimulated human T cells. Flow Cytometry: 25 ug/ml, add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in 200ul. ELISA: 0.5-1 ug/ml, limit ~3 ng/well. Western Blot: 1-2 ug/ml, limit ~25 ng/lane.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute in 200ul sterile PBS.

Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O75144 NCBI: AF289028 AAG01176.1