Yeast Alpha Tubulin

Rat

Yeast (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

YL1/2

Unconjugated

Affinity purified

Unmodified

Yeast tubulin.

C-Terminus

Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.

• IHC
• IHC - Paraffin (5 µg/ml)
• IHC - Frozen
• Immunofluorescence
• Western blot
• Immunoprecipitation
• ELISA (1:100 - 1:1000)
• Radioimmunoassay

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

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