Human Alpha Tubulin

Rabbit

Human, Mouse, Rat, Bovine, Chicken (tested or 100% immunogen sequence identity)

Fish, Algae, Avian, Bacteria, Mammal, Protozoa (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.

aa425-451

A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Immunofluorescence (1:500 - 1:2000)
• Western blot (1:1000 - 1:5000)
• ELISA (1:90000)

Immunohistochemistry: LS-B722 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B722 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.