Human Alpha Tubulin

Rabbit

Human, Mouse, Rat, Bovine, Goat, Sheep, Chicken (tested or 100% immunogen sequence identity)

Fish, Algae, Avian, Bacteria, Mammal, Protozoa (at least 90% immunogen sequence identity)

IgG Polyclonal

Horseradish Peroxidase

Affinity purified

Unmodified

Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.

aa425-451

A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

• IHC (1:500 - 1:2500)
• Western blot (1:1000 - 1:5000)
• ELISA (1:20000)

Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kD in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide

Reconstitute in 100 µL of deionized water.

Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.