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Catalog Number Size Price
LS-B14484-50 50 µl (1 mg/ml) $460 
ACTB / Beta Actin Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
ACTB / Beta Actin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
ACTB / Beta Actin Antibody - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
ACTB / Beta Actin Antibody - Detection of Human and Mouse Cytoskeletal Actin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Cytoskeletal Actin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
ACTB / Beta Actin Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
ACTB / Beta Actin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
ACTB / Beta Actin Antibody - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
ACTB / Beta Actin Antibody - Detection of Human and Mouse Cytoskeletal Actin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Cytoskeletal Actin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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IHC‑plus™ Polyclonal Rabbit anti‑Human ACTB / Beta Actin Antibody (N‑Terminus, IHC, WB) LS‑B14484

IHC‑plus™ Polyclonal Rabbit anti‑Human ACTB / Beta Actin Antibody (N‑Terminus, IHC, WB) LS‑B14484

Note: This antibody replaces LS-C287381
Antibody:
ACTB / Beta Actin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B14484-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ACTB / Beta Actin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Actin antibody LS-B14484 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (N-Terminus) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ACTB / Beta Actin
Synonyms
ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Orangutan, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Hamster-Golden Syrian, Xenopus tropicalis (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 60 corresponding to amino acids near the N-Terminus of UniprotID: P60709
Epitope
N-Terminus
Specificity
The epitope recognized by A300-491A maps to the N-terminus of human Beta-actin using the numbering given in Swiss-Prot entry P60709 (GeneID 60).
Applications
  • IHC
  • IHC - Paraffin (1:500 - 1:2000) & (5 µg/ml)
  • Western blot (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • Immunoprecipitation
Usage
IHC: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTB / Beta Actin
P60709 NM_001101 NP_001092.1

Validation

ACTB / Beta Actin Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

ACTB / Beta Actin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ ACTB / Beta Actin Antibody (N-Terminus) for IHC, WB/Western LS-B14484 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024