Human ACTB / Beta Actin

Rabbit

Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

Beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.

aa350-375

Reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.

• IHC
• IHC - Paraffin (5 µg/ml)
• Immunofluorescence (1:500 - 1:2000)
• Western blot (1:1000 - 1:4000)
• ELISA (1:10000 - 1:40000)

Immunohistochemistry: LS-B323 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B323 was determined to be 5 ug/ml.Antibody has been tested in IHC with human and frog samples and in Western blot with human and mouse samples. It is expected to react with a wide range of species due to sequence homology.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P60709 NCBI: NM_001101 NP_001092.1