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Catalog Number Size Price
LS-B6503-50 50 µg (1 mg/ml) $460 
ACTB / Beta Actin Antibody - Anti-Beta Actin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ACTB / Beta Actin Antibody - Western blot of beta actin in A: human brain (40141), B: mouse brain (40121), C: rat brain (40121), D: human lung (40146) and E: human spleen (40149)tissue lysates using antibody at 0.25 ug/ml.
ACTB / Beta Actin Antibody - Western blot of beta actin in decreasing amounts of human ovary tissue lysate using antibody at 0.25 ug/ml: A) 40 ug, B) 30 ug, C) 20 ug, and D) 10 ug per lane.
ACTB / Beta Actin Antibody - Anti-Beta Actin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ACTB / Beta Actin Antibody - Western blot of beta actin in A: human brain (40141), B: mouse brain (40121), C: rat brain (40121), D: human lung (40146) and E: human spleen (40149)tissue lysates using antibody at 0.25 ug/ml.
ACTB / Beta Actin Antibody - Western blot of beta actin in decreasing amounts of human ovary tissue lysate using antibody at 0.25 ug/ml: A) 40 ug, B) 30 ug, C) 20 ug, and D) 10 ug per lane.
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IHC‑plus™ Polyclonal Rabbit anti‑Human ACTB / Beta Actin Antibody (aa2‑16, IHC, WB) LS‑B6503

IHC‑plus™ Polyclonal Rabbit anti‑Human ACTB / Beta Actin Antibody (aa2‑16, IHC, WB) LS‑B6503

Note: This antibody replaces LS-C19378
Antibody:
ACTB / Beta Actin Rabbit anti-Human Polyclonal (aa2-16) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B6503-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ACTB / Beta Actin Rabbit anti-Human Polyclonal (aa2-16) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Actin antibody LS-B6503 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (aa2-16) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ACTB / Beta Actin
Synonyms
ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Insect (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Amino acids 2-15 (CDDDIAALVIDNGSG) of human beta actin were used as the immunogen. Percent identity by BLAST analysis: Seq squirt, Sea anemone (100%); Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Medaka, Mosquito, Ant (93%).
Epitope
aa2-16
Specificity
The NCBI accession number for actin is AAA51567. This can not be used to test heart or muscle lysate loading.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.25 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTB / Beta Actin
P60709 NM_001101 NP_001092.1

Validation

ACTB / Beta Actin Antibody - Anti-Beta Actin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Beta Actin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Beta Actin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ ACTB / Beta Actin Antibody (aa2-16) for IHC, WB/Western LS-B6503 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024