Human IGFBP3

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Biotin. Also available Unconjugated.

Immunoaffinity purified

Unmodified

E.coli derived recombinant Human IGF-BP3.

Human IGF-BP3

• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.25 - 1 µg/ml)
• Applications tested for the base form of this product only

ELISA: To detect hIGF-BP3 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP3. To detect hIGF-BP3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IGF-BP3 (LS-B7473) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP3. Western Blot: To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS, pH 7.2

Sterile PBS containing 0.1% BSA

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P17936 NCBI: NM_000598 NP_000589.2