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Catalog Number	Size	Price
LS-C407922-10	10 µg	$318
LS-C407922-100	100 µg	$470

IGFBP3 Antibody - IGFBP3 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.

IGFBP3 Antibody - Western blot analysis of IGFBP-3 using anti-IGFBP-3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat PC-12 whole cell lysates. Lane 5: human U-87MG whole cell lysates. Lane 6: mouse kidney tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP-3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP-3 at approximately 40KD. The expected band size for IGFBP-3 is at 32KD.

IGFBP3 Antibody - IGFBP3 antibody Western blot. All lanes: Anti IGFBP3 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: SGC Whole Cell Lysate at 40 ug. Lane 4: 22RV1 Whole Cell Lysate at 40 ug. Predicted band size: 31 kD. Observed band size: 31 kD.

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Polyclonal Rabbit anti‑Human IGFBP3 Antibody (aa214‑252, IHC, WB) LS‑C407922

Name: Anti-IGFBP3 Antibody | Rabbit anti-Human Polyclonal ELISA,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407922-10
Price: 318.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

IGFBP3 Rabbit anti-Human Polyclonal (aa214-252) Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

$318

LS-C407922-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

IGFBP3 Rabbit anti-Human Polyclonal (aa214-252) Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

IGFBP3 antibody LS-C407922 is an unconjugated rabbit polyclonal antibody to IGFBP3 (aa214-252) from human. It is reactive with human and rat. Validated for ELISA, IHC and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human IGFBP3

Synonyms

See All IGFBP3 Antibodies

Host

Rabbit

Reactivity

Human, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of human IGFBP-3 (214-252 aa RREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCR), identical to the related mouse and rat sequences.

Epitope

aa214-252

Specificity

Expressed by most tissues. Present in plasma.

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
ELISA (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IGFBP3

Uniprot: P17936 NCBI: NM_000598 NP_000589.2

LSBio Ratings

IGFBP3 Antibody (aa214-252) for IHC, WB/Western, ELISA LS-C407922 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

EZH1 and EZH2 promote skeletal growth by repressing inhibitors of chondrocyte proliferation and hypertrophy. Lui JC, Garrison P, Nguyen Q, Ad M, Keembiyehetty C, Chen W, Jee YH, Landman E, Nilsson O, Barnes KM, Baron J. Nature communications. 2016 7:13685. (WB; Human)

Full Text Article PubMed: 27897169

Submit a Publication for LS-C407922