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Catalog Number	Size	Price
LS-C800634-100	100 µl (1 mg/ml)	$379
LS-C800634-200	200 µl (1 mg/ml)	$421

HSPA5 / GRP78 / BiP Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

HSPA5 / GRP78 / BiP Antibody - Staining COS7 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

HSPA5 / GRP78 / BiP Antibody - Western blot analysis on COS7 cell lysates using GRP78 antibody. The lane on the left is treated with the antigen-specific peptide.

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Polyclonal Rabbit anti‑Human HSPA5 / GRP78 / BiP Antibody (IHC, IF, WB) LS‑C800634

Name: Anti-HSPA5 / GRP78 / BiP Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C800634-100
Price: 379.0000 USD
Availability: InStock

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Antibody:

HSPA5 / GRP78 / BiP Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Rat

Format:

Unconjugated, Unmodified

$379

LS-C800634-100

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

HSPA5 / GRP78 / BiP Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

BiP antibody LS-C800634 is an unconjugated rabbit polyclonal antibody to BiP (HSPA5 / GRP78) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB.

Target

Human HSPA5 / GRP78 / BiP

Synonyms

HSPA5 | BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78

See All HSPA5 / GRP78 / BiP Antibodies

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Peptide affinity chromatography.

Modifications

Unmodified

Immunogen

A synthesized peptide derived from human GRP78.

Specificity

GRP78 Antibody detects endogenous levels of GRP78.

Applications

IHC (1:50 - 1:200)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:2000)
Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.

Presentation

PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About HSPA5 / GRP78 / BiP