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Antibodies > HNF4A / HNF4 > LS-C801090
 
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Catalog Number Size Price
LS-C801090-100 100 µl (1 mg/ml) $379 
LS-C801090-200 200 µl (1 mg/ml) $421 
HNF4A / HNF4 Antibody - 1:100 staining human kidney carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HNF4A / HNF4 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HNF4A / HNF4 Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
HNF4A / HNF4 Antibody - Western blot analysis of HNF4 alpha phosphorylation expression in EGF treated HuvEc whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
HNF4A / HNF4 Antibody - Western blot analysis of Phospho-HNF4 alpha (Ser313) expression in various lysates
HNF4A / HNF4 Antibody - 1:100 staining human kidney carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HNF4A / HNF4 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HNF4A / HNF4 Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
HNF4A / HNF4 Antibody - Western blot analysis of HNF4 alpha phosphorylation expression in EGF treated HuvEc whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
HNF4A / HNF4 Antibody - Western blot analysis of Phospho-HNF4 alpha (Ser313) expression in various lysates
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Polyclonal Rabbit anti‑Human HNF4A / HNF4 Antibody (phospho‑Ser313, IHC, IF, WB) LS‑C801090

Polyclonal Rabbit anti‑Human HNF4A / HNF4 Antibody (phospho‑Ser313, IHC, IF, WB) LS‑C801090

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Antibody:
HNF4A / HNF4 Rabbit anti-Human Polyclonal (pSer313) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801090-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HNF4A / HNF4 Rabbit anti-Human Polyclonal (pSer313) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HNF4 antibody LS-C801090 is an unconjugated rabbit polyclonal antibody to HNF4 (HNF4A) (pSer313) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human HNF4A / HNF4
Synonyms
HNF4A | Hepatic nuclear factor 4 alpha | HNF4 | HNF4a8 | NR2A21 | TCF | TCF-14 | TCF14 | MODY1 | Transcription factor 14 | Transcription factor HNF-4 | HNF-4-alpha | HNF4 alpha | HNF4a7 | HNF4a9 | HNF4alpha | HNF4alpha10/11/12 | MODY | NR2A1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human HNF4 alpha around the phosphorylation site of Serine 313.
Epitope
pSer313
Specificity
Phospho-HNF4 alpha (Ser313) Antibody detects endogenous levels of HNF4 alpha only when phosphorylated at Serine 313.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HNF4A / HNF4
P41235 NM_000457 NP_000448.3

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Featured Products

HNF4A / HNF4 Antibody - Anti-HNF4A / HNF4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HNF4A / HNF4 Antibody (N‑Terminus) LS‑B969
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot
HNF4A / HNF4 Antibody - Anti-HNF4A / HNF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
IHC‑plus™ HNF4A / HNF4 Antibody (aa2‑15) LS‑B3005
Species: Human, Monkey, Bat, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
HNF4A / HNF4 Antibody - Anti-HNF4A / HNF4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HNF4A / HNF4 Antibody (C‑Terminus) LS‑B4568
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
HNF4A / HNF4 Antibody - HNF4A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
HNF4A / HNF4 Antibody (aa281‑312) LS‑C97258
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Product
HNF4A / HNF4 Antibody (Biotin) LS‑C522411
Species: Human, Mouse
Applications: Western blot
HNF4A / HNF4 Antibody - Confocal immunofluorescence of HNF4A Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
HNF4A / HNF4 Antibody (aa107‑139) LS‑C164947
Species: Human
Applications: Immunofluorescence, Western blot
See all HNF4A / HNF4 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/2/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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