Human HNF4A / HNF4

Goat

Human, Monkey, Bat, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence RLSKTLVDMDMADY-C, from the N-Terminus of protein sequence according to NP_849180.1NP_000448.3NP_849181.1.

aa2-15

Human HNF4A / HNF4. This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).

• IHC
• IHC - Paraffin (2 µg/ml)
• Western blot (0.1 - 0.5 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:32000)

Immunohistochemistry: LS-B3005 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3005 was determined to be 2 ug/ml.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P41235 NCBI: NM_000457 NP_000448.3