Human HNF4A / HNF4

Rabbit

Human, Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin, FITC, HRP.

Immunoaffinity purified

Unmodified

Synthetic peptide from N-Terminus of human HNF4A (P41235, NP_000448). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard (100%); Catfish, Stickleback, Zebrafish (92%); Xenopus (85%).

N-Terminus

Human HNF4A / HNF4, immunogenic peptide sequence is present in isoforms 1, 2, 3, and 4.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (0.2 - 1 µg/ml)

Immunohistochemistry: LS-B969 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B969 was determined to be 5 ug/ml.

PBS, 0.09% sodium azide, 2% sucrose

Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P41235 NCBI: NM_000457 NP_000448.3