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Catalog Number Size Price
LS-C808649-100 100 µl (1 mg/ml) $379 
LS-C808649-200 200 µl (1 mg/ml) $421 
HNF4A / HNF4 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HNF4A / HNF4 Antibody - Western blot analysis of HNF4 alpha expression in HT29 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
HNF4A / HNF4 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HNF4A / HNF4 Antibody - Western blot analysis of HNF4 alpha expression in HT29 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human HNF4A / HNF4 Antibody (IF, WB) LS‑C808649

Polyclonal Rabbit anti‑Human HNF4A / HNF4 Antibody (IF, WB) LS‑C808649

Antibody:
HNF4A / HNF4 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C808649-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HNF4A / HNF4 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HNF4 antibody LS-C808649 is an unconjugated rabbit polyclonal antibody to HNF4 (HNF4A) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human HNF4A / HNF4
Synonyms
HNF4A | Hepatic nuclear factor 4 alpha | HNF4 | HNF4a8 | NR2A21 | TCF | TCF-14 | TCF14 | MODY1 | Transcription factor 14 | Transcription factor HNF-4 | HNF-4-alpha | HNF4 alpha | HNF4a7 | HNF4a9 | HNF4alpha | HNF4alpha10/11/12 | MODY | NR2A1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human HNF4 alpha.
Specificity
HNF4 alpha Antibody detects endogenous levels of total HNF4 alpha.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HNF4A / HNF4
P41235 NM_000457 NP_000448.3

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Requested From: United States
Date Requested: 12/2/2024