Target
Mouse HNF4A / HNF4
Synonyms
HNF4A | Hepatic nuclear factor 4 alpha | HNF4 | HNF4a8 | NR2A21 | TCF | TCF-14 | TCF14 | MODY1 | Transcription factor 14 | Transcription factor HNF-4 | HNF-4-alpha | HNF4 alpha | HNF4a7 | HNF4a9 | HNF4alpha | HNF4alpha10/11/12 | MODY | NR2A1
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-HPHLMQEHMGTN, from the internal region of the protein sequence according to NP_849180.1NP_000448.3NP_787110.2NP_001025174.1NP_001245284.1NP_001274111.1NP_001274112.1.
Specificity
This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9
Applications
- Immunofluorescence (10 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments showed an approx 55kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8kDa according to NP_000448.3). Recommended concentration:0.3-1µg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10µg/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee