Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C758303-100 100 µg (0.5 mg/ml) $503 
HNF4A / HNF4 Antibody - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing a low level of nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Polyclonal Goat anti‑Mouse HNF4A / HNF4 Antibody (IF) LS‑C758303

Polyclonal Goat anti‑Mouse HNF4A / HNF4 Antibody (IF) LS‑C758303

Antibody:
HNF4A / HNF4 Goat anti-Mouse Polyclonal Antibody
Application:
IF, Peptide-ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C758303-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HNF4A / HNF4 Goat anti-Mouse Polyclonal Antibody
Application:
IF, Peptide-ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
HNF4 antibody LS-C758303 is an unconjugated goat polyclonal antibody to mouse HNF4 (HNF4A). Validated for IF and Peptide-ELISA. Cited in 1 publication.
Target
Mouse HNF4A / HNF4
Synonyms
HNF4A | Hepatic nuclear factor 4 alpha | HNF4 | HNF4a8 | NR2A21 | TCF | TCF-14 | TCF14 | MODY1 | Transcription factor 14 | Transcription factor HNF-4 | HNF-4-alpha | HNF4 alpha | HNF4a7 | HNF4a9 | HNF4alpha | HNF4alpha10/11/12 | MODY | NR2A1
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-HPHLMQEHMGTN, from the internal region of the protein sequence according to NP_849180.1NP_000448.3NP_787110.2NP_001025174.1NP_001245284.1NP_001274111.1NP_001274112.1.
Specificity
This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9
Applications
  • Immunofluorescence (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments showed an approx 55kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8kDa according to NP_000448.3). Recommended concentration:0.3-1µg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10µg/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HNF4A / HNF4
P41235 NM_000457 NP_000448.3

LSBio Ratings

HNF4A / HNF4 Antibody for IF/Immunofluorescence LS-C758303 has an LSBio Rating of
Publications (4)

Publications (1)

Endothelin Receptor-A Inhibition Decreases Ductular Reaction, Liver Fibrosis, and Angiogenesis in a Model of Cholangitis. Travis Owen, Guido Carpino, Lixian Chen, Debjyoti Kundu, Payton Wills, Burcin Ekser, Paolo Onori, Eugenio Gaudio, Gianfranco Alpini, Heather Francis, Lindsey Kennedy. Cellular and molecular gastroenterology and hepatology. 2023 June;16:513-540.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/2/2024