Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C670393-50 50 µl $298 
LS-C670393-100 100 µl $370 
H2AFX / H2AX Antibody - Immunofluorescence staining of hela with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
H2AFX / H2AX Antibody - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. All lanes: H2AFX antibody at 1.8 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa. Observed band size: 16 KDa
H2AFX / H2AX Antibody - Chromatin Immunoprecipitation Hela (4*10E6) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFX (Phospho-H2AFX (S139) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
H2AFX / H2AX Antibody - Immunofluorescence staining of hela with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
H2AFX / H2AX Antibody - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. All lanes: H2AFX antibody at 1.8 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa. Observed band size: 16 KDa
H2AFX / H2AX Antibody - Chromatin Immunoprecipitation Hela (4*10E6) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFX (Phospho-H2AFX (S139) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (phospho‑Ser139, IF, WB) LS‑C670393

Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (phospho‑Ser139, IF, WB) LS‑C670393

Antibody:
H2AFX / H2AX Rabbit anti-Human Polyclonal (pSer139) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$298
LS-C670393-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
H2AFX / H2AX Rabbit anti-Human Polyclonal (pSer139) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
H2AX antibody LS-C670393 is an unconjugated rabbit polyclonal antibody to human H2AX (H2AFX) (pSer139). Validated for ELISA, IF and WB.
Target
Human H2AFX / H2AX
Synonyms
H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Epitope
pSer139
Applications
  • Immunofluorescence
  • Western blot
  • ELISA
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About H2AFX / H2AX
P16104 NM_002105 NP_002096.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/30/2024