Drosophila GSK3A / GSK3 Alpha

Mouse

Drosophila, Insect (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

Synthetic peptide corresponding to aa203-219 (18aa, C-KQLLHGEPNVSYICSRY) derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK-3/ shaggy enzyme (ovalbumin coupled). Percent identity by BLAST analysis: Drosophila, Mosquito (100%); Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (88%).

aa203-219

Recognizes Glycogen Synthase Kinase 3 (GSK3). Due to extremely high conservation among the known enzymes of many different species, should cross-react with nearly all species homologues. Species cross-reactivity: Mouse, rat, S. pombe, Dictyostelium and human.

• ICC (10 µg/ml)
• Western blot (0.5 - 2 µg/ml)

• Immunoprecipitation

WB: Detects GSK-3 in RIPA lysates from human Jurkat and A431, rat L6 and PC12 and mouse 3T3 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with Mouse Anti-Glycogen Synthase Kinase 3 (0.5 ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. ICC: Demonstrated positive immunostaining for GSK3 in L6 cells fixed with acetone.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P49840 NCBI: NM_019884 NP_063937.2