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Catalog Number Size Price
LS-C344899-50 50 µg $337 
LS-C344899-100 100 µg $386 
FTO Antibody - Immunoprecipitation of recombinant human FTO using anti-FTO (human), mAb (FT86-4). Recombinant human FTO proteins at different concentrations were precipitated by FT86-4. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with rabbit anti-mouse FTO pAb.
FTO Antibody - Western blot analysis using anti-FTO (human), mAb (FT86-4) at 1:2000 dilution. 1: Human FTO (His-tagged) (100 ng). 2: Mouse FTO (His-tagged) (100ng). 3: Rat FTO (His-tagged) (100 ng). 4: HEK 293 cell lysate (100 ug). 5: Human Calreticulin (His-tagged) (100ng) (negative control).
FTO Antibody - Immunoprecipitation of recombinant human FTO using anti-FTO (human), mAb (FT86-4). Recombinant human FTO proteins at different concentrations were precipitated by FT86-4. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with rabbit anti-mouse FTO pAb.
FTO Antibody - Western blot analysis using anti-FTO (human), mAb (FT86-4) at 1:2000 dilution. 1: Human FTO (His-tagged) (100 ng). 2: Mouse FTO (His-tagged) (100ng). 3: Rat FTO (His-tagged) (100 ng). 4: HEK 293 cell lysate (100 ug). 5: Human Calreticulin (His-tagged) (100ng) (negative control).
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2 of 2

Monoclonal Mouse anti‑Human FTO Antibody (clone FT86‑4, WB) LS‑C344899

Monoclonal Mouse anti‑Human FTO Antibody (clone FT86‑4, WB) LS‑C344899

Antibody:
FTO Mouse anti-Human Monoclonal (FT86-4) Antibody
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$337
LS-C344899-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
FTO Mouse anti-Human Monoclonal (FT86-4) Antibody
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
FTO antibody LS-C344899 is an unconjugated mouse monoclonal antibody to FTO from human. It is reactive with human, mouse and rat. Validated for ELISA, IP and WB.
Target
Human FTO
Synonyms
FTO | KIAA1752 | Protein fto
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
FT86-4
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Recombinant human FTO.
Specificity
Recognizes human, mouse and rat FTO.
Applications
  • Western blot (1:2000 - 1:5000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:5000)
Usage
ELISA - Direct or indirect: 1:2000-1:5000. Immunoprecipitation - 1-5ul per sample in a final reaction volume of 500-1000ul. Western blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FTO
Q9C0B1 Q9C0B1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2024