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Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
FTO antibody LS-C344899 is an unconjugated mouse monoclonal antibody to FTO from human. It is reactive with human, mouse and rat. Validated for ELISA, IP and WB.
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1,kMonoclonal
Clone
FT86-4
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Recombinant human FTO.
Specificity
Recognizes human, mouse and rat FTO.
Applications
Western blot (1:2000 - 1:5000)
Immunoprecipitation
ELISA (1:2000 - 1:5000)
Usage
ELISA - Direct or indirect: 1:2000-1:5000. Immunoprecipitation - 1-5ul per sample in a final reaction volume of 500-1000ul. Western blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.