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Catalog Number	Size	Price
LS-C344911-50	50 µg	$354
LS-C344911-100	100 µg (1 mg/ml)	$407

FTO Antibody - Immunohistochemical staining of mouse FTO using anti-FTO (mouse), mAb (FT62-6) in mouse tissue (1:500 dilution, 200X). A. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse brain. A-1. Isotype control, mouse brain. B. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse uterus. B-1. Isotype control, mouse uterus.

FTO Antibody - Western blot analysis using anti-FTO (mouse), mAb (FT62-6) at 1:2000 dilution. A). 1: Mouse kidney (200 ug). 2: Mouse spleen (200 ug). 3: Mouse heart (200 ug). 4: Mouse liver (500 ug). 5: Mouse stomach (200 ug). 6: Mouse brain (300 ug). 7: Mouse lung (200 ug). B). 1: Mouse FTO (10ng). 2: 3T3L1 (100 ug).

FTO Antibody - Immunoprecipitation confirmed by recombinant mouse FTO. Recombinant mouse FTO proteins at different concentrations were precipitated using anti-FTO (mouse), mAb (FT62-6) . The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with rabbit anti-mouse FTO pAb. 1. mFTO (His-tagged) recombinant control 50ng (AG-40A-127). 2. mFTO (His-tagged) 10 ug. 3. mFTO (His-tagged) 5 ug. 4. mFTO (His-tagged) 2.5 ug. 5. mFTO (His-tagged) 1.25 ug. 6. mFTO (His-tagged) 0.312 ug. 7. mFTO (His-tagged) 0.156 ug.

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Monoclonal Mouse anti‑Mouse FTO Antibody (clone FT62‑6, IHC, WB) LS‑C344911

Name: Anti-FTO Antibody | Mouse anti-Mouse Monoclonal ELISA,IHC,IP,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C344911-50
Price: 354.0000 USD
Availability: InStock

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Antibody:

FTO Mouse anti-Mouse Monoclonal (FT62-6) Antibody

Application:

IHC, IHC-P, WB, IP, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

$354

LS-C344911-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

FTO Mouse anti-Mouse Monoclonal (FT62-6) Antibody

Application:

IHC, IHC-P, WB, IP, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

FTO antibody LS-C344911 is an unconjugated mouse monoclonal antibody to mouse FTO. Validated for ELISA, IHC, IP and WB.

Target

Mouse FTO

Synonyms

FTO | KIAA1752 | Protein fto

See All FTO Antibodies

Host

Mouse

Reactivity

Mouse (tested or 100% immunogen sequence identity)

Clonality

IgG1,k Monoclonal

Clone

FT62-6

Conjugations

Unconjugated

Purification

Protein G purified from tissue culture supernatant

Modifications

Unmodified

Immunogen

Recombinant human FTO.

Specificity

Recognizes mouse FTO. Weakly cross-reacts with human FTO. Does not cross-react with rat FTO.

Applications

IHC
IHC - Paraffin (1:100 - 1:500)
Western blot (1:2000 - 1:5000)
Immunoprecipitation (5 µg/ml)
ELISA (1:2000 - 1:5000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

ELISA - Direct and indirect: 1:2000-1:5000. Immunohistochemistry - Paraffin sections (1:100-1:500. Immunoprecipitation - 5 ug/ml per sample in a final reaction volume of 500-1000ul. Western Blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.

Presentation

PBS, pH 7.4, no preservatives.

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About FTO

Uniprot: Q9C0B1 NCBI: Q9C0B1