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Catalog Number Size Price
LS-C154234-100 100 µg (1.1 mg/ml) $567 
FAP Alpha Antibody - Anti-FAP Antibody - Western Blot. Western blot of affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kD, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1000. Personal communication, S. Kim, NCI, Bethesda, MD.

Polyclonal Rabbit anti‑Mouse FAP Alpha Antibody (WB) LS‑C154234

Polyclonal Rabbit anti‑Mouse FAP Alpha Antibody (WB) LS‑C154234

Antibody:
FAP Alpha Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C154234-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
FAP Alpha Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
FAP Alpha antibody LS-C154234 is an unconjugated rabbit polyclonal antibody to mouse FAP Alpha. Validated for ELISA and WB.
Target
Mouse FAP Alpha
Synonyms
FAP | DPPIV | FAP alpha | Fibroblast activation protein | FAPA | Seprase
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.
Specificity
This antibody is known to react with mouse FAP protein. A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88% homology with the immunizing sequence.  Reactivity with FAP from other sources has not been determined.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:20000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 88 kD in size corresponding to FAP by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FAP Alpha
Q12884 NM_004460 NP_004451.2

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Requested From: United States
Date Requested: 11/23/2024