Target
Human FAP Alpha
Synonyms
FAP | DPPIV | FAP alpha | Fibroblast activation protein | FAPA | Seprase
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Purified, Sf 21-derived, recombinant human FAP (rhFAP; aa 26-760; Accession# Q12884).
Specificity
Recognizes human Fibroblast Activation Protein alpha. Does not cross-react with recombinant human DPP6.
Applications
- Flow Cytometry (25 µg/ml)
- ELISA (2 - 8 µg/ml)
Usage
Suitable for use in Flow Cytometry and ELISA. Flow Cytometry: 25 ug/ml. 10 ul of the diluted solution labels 1-2.5x10^5 WI-38 cells in a total reaction volume 200ul. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as an IgG (FITC) anti-mouse. ELISA: 2-8 ug/ml. Can be used in combination with biotinylated human FAP detection antibody and recombinant human FAP as the standard.
Presentation
PBS, pH 7.4, 5% Trehalose
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee