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Catalog Number	Size	Price
LS-C413062-100	100 µl (1 mg/ml)	$321

EP300 / p300 Antibody - Immunohistochemical analysis of p300 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

EP300 / p300 Antibody - Immunofluorescent analysis of p300 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

EP300 / p300 Antibody - Western blot analysis of p300 expression in HeLa (A); HEK293T (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human EP300 / p300 Antibody (N‑Terminus, IHC, IF, WB) LS‑C413062

Name: Anti-EP300 / p300 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C413062-100
Price: 321.0000 USD
Availability: InStock

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Antibody:

EP300 / p300 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$321

LS-C413062-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

EP300 / p300 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

P300 antibody LS-C413062 is an unconjugated rabbit polyclonal antibody to p300 (EP300) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.

Target

Human EP300 / p300

Synonyms

See All EP300 / p300 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human p300.

Epitope

N-Terminus

Specificity

Recognizes endogenous levels of p300 protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About EP300 / p300

Uniprot: Q09472 NCBI: NM_001429 NP_001420.2