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Catalog Number Size Price
LS-C344035-10 10 µg $318 
LS-C344035-100 100 µg (0.5 mg/ml) $470 
EP300 / p300 Antibody - KAT3B/p300 antibody IHC-paraffin: Human Esophagus Squamous Cancer Tissue.
EP300 / p300 Antibody - KAT3B/p300 antibody IHC-paraffin: Rat Intestine Tissue.
EP300 / p300 Antibody - KAT3B/p300 antibody IHC-paraffin: Mouse Intestine Tissue.
EP300 / p300 Antibody - Western blot analysis of KAT3B/p300 using anti-KAT3B/p300 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT3B/p3008 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KAT3B/p300 at approximately 300KD. The expected band size for KAT3B/p300 is at 264KD.
EP300 / p300 Antibody - KAT3B/p300 antibody Western blot. All lanes: Anti KAT3B/p300 at 0.5 ug/ml. Lane 1: Colo320 Whole Cell Lysate at 40 ug. Lane 2: PC12 Whole Cell Lysate at 40 ug. Predicted band size: 264 kD. Observed band size: 264 kD.
EP300 / p300 Antibody - KAT3B/p300 antibody Western blot. All lanes: All lanes: Anti KAT3B/p300 at 0.5 ug/ml. WB: Recombinant Human KAT3B/p300 Protein 0.5ng. Predicted band size: 50 kD. Observed band size: 50 kD.
EP300 / p300 Antibody - KAT3B/p300 antibody IHC-paraffin: Human Esophagus Squamous Cancer Tissue.
EP300 / p300 Antibody - KAT3B/p300 antibody IHC-paraffin: Rat Intestine Tissue.
EP300 / p300 Antibody - KAT3B/p300 antibody IHC-paraffin: Mouse Intestine Tissue.
EP300 / p300 Antibody - Western blot analysis of KAT3B/p300 using anti-KAT3B/p300 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT3B/p3008 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KAT3B/p300 at approximately 300KD. The expected band size for KAT3B/p300 is at 264KD.
EP300 / p300 Antibody - KAT3B/p300 antibody Western blot. All lanes: Anti KAT3B/p300 at 0.5 ug/ml. Lane 1: Colo320 Whole Cell Lysate at 40 ug. Lane 2: PC12 Whole Cell Lysate at 40 ug. Predicted band size: 264 kD. Observed band size: 264 kD.
EP300 / p300 Antibody - KAT3B/p300 antibody Western blot. All lanes: All lanes: Anti KAT3B/p300 at 0.5 ug/ml. WB: Recombinant Human KAT3B/p300 Protein 0.5ng. Predicted band size: 50 kD. Observed band size: 50 kD.
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Polyclonal Rabbit anti‑Human EP300 / p300 Antibody (aa2065‑2414, IHC, WB) LS‑C344035

Polyclonal Rabbit anti‑Human EP300 / p300 Antibody (aa2065‑2414, IHC, WB) LS‑C344035

Note: This antibody replaces LS-C389047
Antibody:
EP300 / p300 Rabbit anti-Human Polyclonal (aa2065-2414) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C344035-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
EP300 / p300 Rabbit anti-Human Polyclonal (aa2065-2414) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
P300 antibody LS-C344035 is an unconjugated rabbit polyclonal antibody to p300 (EP300) (aa2065-2414) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human EP300 / p300
Synonyms
EP300 | E1A binding protein p300 | Histone acetyltransferase p300 | KAT3B | p300 HAT | E1A-associated protein p300 | E1A-binding protein, 300kD | p300 | RSTS2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human KAT3B recombinant protein (Position: L2065-H2414). Human KAT3B shares 94% amino acid (aa) sequence identity with mouse KAT3B.
Epitope
aa2065-2414
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EP300 / p300
Q09472 NM_001429 NP_001420.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/13/2024