Human CTLA4 / CD152

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

ANC152.2/8H5

FITC. Also available Unconjugated or conjugated with Biotin, RPE.

Protein A purified

Unmodified

Mouse IgG1, kappa.

Antibody ANC152.2/8H5 reacts with an extracellular epitope of human CTLA-4 expressed on activated T cells.

• Flow Cytometry
• ELISA
• Blocking
• Applications tested for the base form of this product only

Anti-CD152/FITC was tested for binding to CD152-muIg-coated 4u beads in FACS (Inquire). Two x 105 CD152-muIg coated beads per tube were spun down and incubated 45 minutes at 4oC with 80ul of anti-CD152/FITC at a 1:50 dilution (10ug/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 2.03 log10 fluorescent units when compared to a Mouse IgG1/FITC Isotype control at a similar concentration. Binding was blocked when beads were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD152 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA

Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16410 NCBI: NM_005214 NP_005205.2