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Catalog Number Size Price
LS-C756401-10 10 µg $318 
LS-C756401-100 100 µg $470 
COMT Antibody - Western blot analysis of COMT using anti-COMT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates,Lane 2: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COMT antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COMT at approximately 25 and 29KD. The expected band size for COMT is at 29KD.

Polyclonal Rabbit anti‑Mouse COMT Antibody (WB) LS‑C756401

Polyclonal Rabbit anti‑Mouse COMT Antibody (WB) LS‑C756401

Antibody:
COMT Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756401-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
COMT Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
COMT antibody LS-C756401 is an unconjugated rabbit polyclonal antibody to COMT from mouse. It is reactive with mouse and rat. Validated for ELISA and WB.
Target
Mouse COMT
Synonyms
COMT | Catechol O-methyltransferase | Catechol-O-methyltransferase
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse COMT recombinant protein (Position: R20-S265).
Specificity
No cross reactivity with other proteins.
Applications
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COMT
P21964 NM_007310 NP_009294.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024