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Catalog Number	Size	Price
LS-C407752-10	10 µg	$318
LS-C407752-100	100 µg	$470

COMT Antibody - IHC analysis of COMT using anti-COMT antibody. COMT was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-COMT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

COMT Antibody - IHC analysis of COMT using anti-COMT antibody. COMT was detected in frozen section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-COMT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

COMT Antibody - IHC analysis of COMT using anti-COMT antibody. COMT was detected in frozen section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-COMT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

COMT Antibody - COMT antibody IHC-paraffin. IHC(P): Mouse Lung Tissue.

COMT Antibody - COMT antibody IHC-paraffin. IHC(P): Rat Lung Tissue.

COMT Antibody - COMT antibody IHC-paraffin. IHC(P): Human Placenta Tissue.

COMT Antibody - COMT antibody ICC. ICC: A549 Cell.

COMT Antibody - COMT antibody Western blot. All lanes: Anti COMT at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: Rat Kidney Tissue Lysate at 50 ug. Lane 4: Mouse Brain Tissue Lysate at 50 ug. Lane 5: JURKAT Whole Cell Lysate at 40 ug. Lane 6: CEM Whole Cell Lysate at 40 ug. Lane 7: HELA Whole Cell Lysate at 40 ug. Predicted band size: 30 kD. Observed band size: 30 kD.

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Polyclonal Rabbit anti‑Human COMT Antibody (aa52‑271, IHC, WB) LS‑C407752

Name: Anti-COMT Antibody | Rabbit anti-Human Polyclonal ICC,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407752-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

COMT Rabbit anti-Human Polyclonal (aa52-271) Antibody

Application:

IHC, IHC-P, ICC, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C407752-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

COMT Rabbit anti-Human Polyclonal (aa52-271) Antibody

Application:

IHC, IHC-P, ICC, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

COMT antibody LS-C407752 is an unconjugated rabbit polyclonal antibody to COMT (aa52-271) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.

Target

Human COMT

Synonyms

COMT | Catechol O-methyltransferase | Catechol-O-methyltransferase

See All COMT Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human COMT recombinant protein (Position: G52-P271). Human COMT shares 81.9% and 81% amino acid (aa) sequence identity with mouse and rat COMT, respectively.

Epitope

aa52-271

Specificity

Brain, liver, placenta, lymphocytes and erythrocytes.

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
ICC (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About COMT

Uniprot: P21964 NCBI: NM_007310 NP_009294.1