Chicken Collagen V

Rabbit

Chicken (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Unmodified

Purified collagen type V from chicken skin

• IHC - Paraffin (1:200 - 1:600)
• Immunofluorescence (1:40)
• ELISA
• Radioimmunoassay

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Collagen type V is found as short fibrils in skin, bone and placenta. Often it is found in conjunction with other collagen types, especially type I and III. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type V 100%, chicken collagen type I, II, III and X <0.1%; chicken fibronectin <0.1%; human collagen type V <0.1%.

PBS

0.5 ml Reconstitute in sterile distilled water.

Store at -20°C.

For research use only. Intended for use by laboratory professionals.

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