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Catalog Number Size Price
LS-C18874-100 100 µg (1 mg/ml) $574 
Collagen V Antibody - Anti-Collagen V Antibody - Immunohistochemistry. anti-collagen V antibody (1:200, 45 min RT) showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA.

Polyclonal Rabbit anti‑Mammal Collagen V Antibody (IHC, WB) LS‑C18874

Polyclonal Rabbit anti‑Mammal Collagen V Antibody (IHC, WB) LS‑C18874

Antibody:
Collagen V Rabbit anti-Mammal Polyclonal Antibody
Application:
IHC, IHC-Fr, WB, IP, ELISA
Reactivity:
Mammal, Human, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$574
LS-C18874-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Collagen V Rabbit anti-Mammal Polyclonal Antibody
Application:
IHC, IHC-Fr, WB, IP, ELISA
Reactivity:
Mammal, Human, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen V antibody LS-C18874 is an unconjugated rabbit polyclonal antibody to Collagen V from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.
Target
Mammal Collagen V
Host
Rabbit
Reactivity
Mammal, Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Collagen Type V from human and bovine placenta.
Specificity
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Applications
  • IHC
  • IHC - Frozen (1:50 - 1:200)
  • Western blot (1:1000 - 1:10000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C for short term applications. For long term storage, diluted with an equal volume of Glycerol and stored at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/13/2024