Dog Collagen V

Mouse

Dog, Human, Sheep (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

Unconjugated

Protein A/G purified

Unmodified

Acid-digested pepsin soluble canine type V collagen.

Highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and Western Blot. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). Shows reactivity with human, canine, bovine, sheep, porcine and kangaroo type V collagens by ELISA and Western Blot. Does not show reactivity with mouse, rat, guinea pig and chicken type V collagens by ELISA and Western Blot. Can be used for detection of collagens by ELISA. Has been used successfully for immunohistology on frozen unfixed sections of human, bovine and dog skin, of fetal bovine cornea, and of new dog tissue associated with a biomaterial implant. If fixation of tissue is required, acetone or ethanol is recommended. Binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.

• IHC
• Western blot
• ELISA (1:50)

Suitable for use in ELISA, Western Blot, Immunohistochemistry. ELISA: 1:50.

PBS, pH 7.4, 15 mM Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.